DNA

Part:BBa_K1199047:Design

Designed by: Tian Wang   Group: iGEM13_UESTC_Life   (2013-09-17)

LinA-RBS-LinB gamma-HCH->2,5-dichloro-2,5-cyclohexadiene-1,4-diol


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 472
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 652
    Illegal NgoMIV site found at 1143
    Illegal NgoMIV site found at 1177
    Illegal AgeI site found at 577
    Illegal AgeI site found at 1243
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there are restriction enzyme sites KpnI and XhoI.


Source

LinA and LinB are from Pseudomonas paucimobilis UT26

References